MB-CasFire™: A Plug-and-Play, Thermostable CRISPR Enzyme for Precise Nucleic Acid Detection

CRISPR-based detection technologies have the potential to enable precise, rapid, and cost-effective diagnostic and biosurveillance solutions by pairing sensitive and specific nucleic acid detection with a simple workflow. Combining isothermal amplification and CRISPR detection in a single reaction as a "one-pot" detection method can meet these needs, but only if the CRISPR enzyme remains active at the temperatures and salt concentrations that isothermal amplification requires. Here we describe MB-CasFire, a CRISPR-Cas enzyme engineered from a naturally thermostable Cas12a for improved stabilit

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