Sporadic detection of dengue virus RNA in California wastewater solids

Dengue virus (DENV) is the most prevalent mosquito-borne virus globally, endemic in over 100 countries and associated with around 40,000 deaths each year. Prior research has shown that DENV RNA can be detected in wastewater, but detection rates vary widely depending on the setting and method used. In this study, we analyzed 1,072 untreated wastewater samples from five treatment plants in California, collected between December 2023 and December 2025, to evaluate wastewater-based surveillance for DENV. We compared wastewater data with reported clinical incidence and Aedes aegypti mosquito abunda

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