Integrating 16S and 18S sequencing reveals coupled prokaryotic and eukaryotic relationships in drinking water biofilms

Drinking water biofilms host diverse communities spanning all realms of life. However, despite the known interactions between opportunistic pathogens and microbial eukaryotes such as amoebae, most studies focus only on the prokaryotic populations in these systems. As a result, the degree to which microbial eukaryotes shape biofilm communities and the persistence of opportunistic waterborne pathogens, such as Legionella spp. is still largely unknown. Here we leveraged a set of 128 non-disinfected drinking water shower hose biofilms collected across Switzerland to characterize both communities a

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