Diagnostic accuracy of a single-reaction, pan-lineage Lassa virus RT-PCR at two Nigerian laboratories: a retrospective and prospective cohort study

Background Lassa virus (LASV) intra- and inter-lineage nucleotide diversity has made it challenging to develop a single-reaction RT-PCR assay that can detect all viral variants with equal sensitivity. We evaluated PANDAA(R) LASV, a single-reaction pan-lineage RT-PCR assay, at two Nigerian Lassa fever laboratories against the RealStar(R) Lassa Virus RT-PCR Kit 2.0, the current diagnostic standard in Nigeria, which requires two separate reactions per patient sample to mitigate against false negative results. Methodology and Principal Findings We conducted a diagnostic accuracy study using retros

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