Cryopreserved Peripheral Blood Mononuclear Cells Recover Mitochondrial Function Following Overnight Culture Revealed by High-Resolution Respirometry

Abstract Background: High resolution respirometry of peripheral blood mononuclear cells (PBMCs) is widely used to assess mitochondrial function, but reliance on freshly isolated cells limits flexibility and sample batching. The impact of cryopreservation and post-thaw handling on PBMC mitochondrial respiration remains incompletely defined. Methods: PBMCs from healthy young adults were analysed under three conditions: freshly isolated, cryopreserved and directly thawed, and cryopreserved followed by overnight recovery in supplemented culture medium. Mitochondrial respiration was assessed using

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