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medRxiv PreprintsInternational15 October 2026

Peak Agricultural Pesticide Spray Seasons Are Associated with worse Negative Affect and Behavioral Dysregulation in Adolescents and Young Adults: A 2-Year Ecological Momentary Assessment Study in ESPINA

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Introduction: Pesticide exposure has been associated with poorer mental health, yet little is known about whether seasonal fluctuations in agricultural pesticide use are associated with cyclical changes in mood among people living in agricultural communities. We evaluated associations between 10 peak and off-peak pesticide spray seasons and ecological momentary assessment (EMA)-derived mood in a floricultural region in Ecuador. Methods: 496 participants completed repeated EMA surveys across 10 assessment administrations over 2.5 years (2022-2024), including one single-assessment administration
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